Novel AAV capsids enhance brain targeting via human CA-IV

CLOVER-affiliated Postdoctoral Fellow Changfan Lin led a team, including current and former CLOVER staff Fiona Ristic, Erin Sullivan, Bill Kavvathas, and Tim Shay, as well as Cal Poly Pomona ASPIRE undergraduate Irene Tran, to develop a hybrid in vitro / in vivo capsid engineering pipeline and a 'humanized' mouse model for vector characterization. Using these, Changfan and the team identify two capsids that target the human version of carbonic anhydrase IV (CA-IV), which was previously identified as the blood-brain barrier transcytosis receptor for some of the most potent AAVs for the mouse brain. Catch the full story in Cell Reports! Congratulations to Changfan and the team!

AAV capsid and mouse 'humanization' plasmids available on Addgene soon!