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  • Characterization and quantification of adeno-associated virus capsid-loading states by multi-wavelength analytical ultracentrifugation with UltraScan. Hendrickson A., Ding X., Seal A.G., Qu Z., Tomlinson L., Forsey J., Gradinaru V., Oka K., Demeler B. Nanomedicine (Lond), 2023; 10.2217/nnm-2023-0156. [ Article ]

  • Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Chuapoco M.R., Flytzanis N.C., Goeden N., Octeau J.C., Roxas K.M., Chan K.Y., Scherrer J., Winchester J., Blackburn R.J., Campos L.J., Mimi Man K.N., Sun J., Chen X., Lefevre A., Singh V.P., Arokiaraj C.M., Shay T.F., Vendemiatti J., Jang M.J., Mich J.K., Bishaw Y., Gore B.B., Omstead V., Taskin N., Weed N., Levi B.P., Ting J.T., Miller C.T., Deverman B.E., Pickel J., Tian L., Fox A.S., Gradinaru V. Nature Nanotechnology, 2023; 10.1038/s41565-023-01419-x. [ Article ]

  • Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates. Chen X., Wolfe D.A., Sivadasan Bindu D., Zhang M., Taskin N., Goertsen D., Shay T.F., Sullivan E.E., Huang S., Ravindra Kumar S., Arokiaraj C.M., Plattner V.M., Campos L.J., Mich J.K., Monet D., Ngo V., Ding X., Omstead V., Weed N., Bishaw Y., Gore B.B., Lein E.S., Akrami A., Miller C., Levi B.P., Keller A., Ting J.T., Fox A.S., Eroglu C., Gradinaru V. Nature Communications, 2023; 10.1038/s41467-023-38582-7. [ Article ]

  • Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors. Shay T.F., Sullivan E.E., Ding X., Chen X., Ravindra Kumar S., Goertsen D., Brown D., Crosby A., Vielmetter J., Borsos M., Wolfe D.A., Lam A.W., Gradinaru V. Science Advances, 2023; 10.1126/sciadv.adg6618. [ Article | Caltech News Release ]

  • CRISPR-clear imaging of melanin-rich B16-derived solid tumors. Schubert R., Bae T., Simic B., Smith S.N., Park S., Nagy-Davidescu G., Gradinaru V., Pluckthun A., Hur J.K. Communications Biology, 2023; 10.1038/s42003-023-04614-7. [ Article ]

  • Diminished neuronal ESCRT-0 function exacerbates AMPA receptor derangement and accelerates prion-induced neurodegeneration. Lawrence J.A., Aguilar-Calvo P., Ojeda-Juarez D., Khuu H., Soldau K., Pizzo D.P., Wang J., Malik A., Shay T.F., Sullivan E.E., Aulston B., Song S.M., Callender J.A., Sanchez H., Geschwind M.D., Roy S., Rissman R.A., Trejo J., Tanaka N., Wu C., Chen X., Patrick G.N., Sigurdson C.J. Journal of Neuroscience, 2023; 10.1523/JNEUROSCI.1878-22.2023. [ Article ]

  • Spatial transcriptomics for profiling the tropism of viral vectors in tissues. Jang M.J., Coughlin G.M., Jackson C.R., Chen X., Chuapoco M.R., Vendemiatti J.L., Wang A.Z., Gradinaru V. Nature Biotechnology, 2023; 10.1038/s41587-022-01648-w. [ Article | Research Briefing ]
  • Molecular basis of astrocyte diversity and morphology across the CNS in health and disease. Endo F., Kasai A., Soto J.S., Qu Z., Hashimoto H., Gradinaru V., Kawaguchi R., Khakh B.S. Science, 2022; https://doi.org/10.1126/science.adc9020. [ Article | Perspective ]

  • Multimodal imaging of capsid and cargo reveals differential brain targeting and liver detargeting of systemically-administered AAVs. Seo J.W., Ajenjo J., Wu B., Robinson E., Nura Raie M., Wang J., Tumbale S.K., Buccino P., Alexander Anders D., Shen B., Habte F.G., Beinat C., James M.L., Taylor Reyes S., Ravindra Kumar S., Miles T.F., Lee J.T., Gradinaru V., Ferrara K.W. Biomaterials, 2022; https://doi.org/10.1016/j.biomaterials.2022.121701. [ Article ]

  • Targeting the lung epithelium after intravenous delivery by directed evolution of underexplored sites on the AAV capsid. Goertsen D., Goeden N., Flytzanis N.C., Gradinaru V. Molecular Therapy Methods Clinical Development, 2022; https://doi.org/10.1016/j.omtm.2022.07.010. [ Article ]

  • Structural basis of receptor usage by the engineered capsid AAV-PHP.eB. Jang S., Shen H.K., Ding X., Miles T.F., Gradinaru V. Molecular Therapy Methods Clinical Development, 2022; https://doi.org/10.1016/j.omtm.2022.07.011. [ Article ]

  • Transduction of systemically administered adeno-associated virus in the colonic enteric nervous system and c-Kit cells of adult mice. Wang L., Yuan P., Challis C., Ravindra Kumar S., Tache Y. Frontiers in Neuroanatomy, 2022; https://doi.org/10.3389/fnana.2022.884280. [ Article ]

  • Engineered AAVs for non-invasive gene delivery to rodent and non-human primate nervous systems. Chen X., Ravindra Kumar S., Adams C.A., Yang D., Wang T., Wolfe D.A., Arokiaraj C.M., Ngo V., Campos L.J., Griffiths J.A., Ichiki T., Mazmanian S.K., Osborne P.B., Keast J.R., Miller C.T., Fox A.S., Chiu I.M., Gradinaru V. Neuron, 2022; https://doi.org/10.1016/j.neuron.2022.05.003. [ Article ]

  • Adeno-associated virus toolkit to target diverse brain cells. Challis R.C., Ravindra Kumar S., Chen X., Goertsen D., Coughlin G.M., Hori A.M., Chuapoco M.R., Otis T.S., Miles T.F., Gradinaru V. Annual Review of Neuroscience, 2022; https://doi.org/10.1146/annurev-neuro-111020-100834. [ Article ]

  • Glutamate in primary afferents is required for itch transmission. Cui L., Guo J., Cranfill S.L., Gautam M., Bhattarai J., Olson W., Beattie K., Challis R.C., Wu Q., Song X., Raabe T., Gradinaru V., Ma M., Liu Q., Luo W. Neuron, 2022; doi: 10.1016/j.neuron.2021.12.007. [ Article ]

  • AAV capsid variants with brain-wide transgene expression and decreased liver targeting after intravenous delivery in mouse and marmoset. Goertsen, D., Flytzanis, N.C., Goeden, N., Chuapoco, M.R., Cummins, A., Chen, Y., Fan, Y., Zhang, Q., Sharma, J., Duan, Y., Wang, L., Feng, G., Chen, Y., Ip, N.Y., Pickel, J., Gradinaru, V. Nature Neuroscience, 2022; doi.org/10.1038/s41593-021-00969-4. [ Article | Caltech News Release ]
  • Deep parallel characterization of AAV Tropism and AAV-mediated transcriptional changes via single-cell RNA sequencing. Brown, D., Altermatt, M., Dobreva, T., Chen, S., Wang, A., Thomson, M., Gradinaru, V. Frontiers in Immunology, 2021; doi: 10.3389/fimmu.2021.730825. [ Article ]

  • Light-guided sectioning for precise in situ localization and tissue interface analysis for brain-implanted optical fibers and GRIN lenses. Kahan, A., Greenbaum, A., Jang, M.J., Robinson, J.E., Cho, J.R., Chen, X., Kassraian, P., Wagenaar, D.A., Gradinaru, V. Cell Reports , 2021; doi: 10.1016/j.celrep.2021.109744. [ Article ]

  • Improved systemic AAV gene therapy with a neurotrophic capsid in Niemann-Pick disease type C1 mice. Davidson, C.D., Gibson, A.L., Gu, T., Baxter, L.L., Deverman, B.E., Beadle, K., Incao, A.A., Rodriguez-Gil, J.L., Fujiwara, H., Jiang, X., Chandler, R.J., Ory, D.S., Gradinaru, V., Venditti, C.P., Pavan, W.J. Life Science Alliance, 2021; doi: 10.26508/lsa.202101040. [ Article ]

  • Brain-wide Cas9-mediated cleavage of a gene causing familial Alzheimer's disease alleviates amyloid-related pathologies in mice. Duan, Y., Ye, T., Qu, Z., Chen, Y., Miranda, A., Zhou, X., Lok, K., Chen, Y., Fu, A.K.Y., Gradinaru, V., Ip, N.Y. Nature Biomedical Engineering, 2021; doi: 10.1038/s41551-021-00759-0. [ Article ]

  • Microbiota regulate social behavior via stress response neurons in the brain. Wu, W.L., Adame, M.D., Liou, C.W., Barlow, J.T., Lai, T.T., Sharon, G., Schretter, C.E., Needham, B.D., Wang, M.I., Tang, W., Ousey, J., Lin, Y.Y., Yao, T.H., Abdel-Haq, R., Beadle, K., Gradinaru, V., Ismagilov, R.F., Mazmanian, S.K. Nature, 2021; doi: 10.1038/s41586-021-03669-y. [ Article ]

  • Specific and behaviorally consequential astrocyte Gq GPCR signaling attenuation in vivo with iβARK. Nagai, J., Bellafard, A., Qu, Z., Yu, X., Ollivier, M., Gangwani, M.R., Diaz-Castro, B., Coppola, G., Schumacher, S.M., Golshani, P., Gradinaru, V., Khakh, B.S. Neuron, 2021; doi: 10.1016/j.neuron.2021.05.023. [ Article ]

  • Use of high-content imaging to quantify transduction of AAV-PHP viruses in the brain following systemic delivery. Smith, E.J., Farshim, P.P., Flomen, R., Jones, S.T., McAteer, S.J., Deverman, B.E., Gradinaru, V., Bates, G.P. Brain Communications, 2021; doi.org/10.1093/braincomms/fcab105 [ Article ]

  • Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Mich J., Graybuck L.T., Hess E.E., Mahoney J.T., Kojima Y., Ding Y., Somasundaram S., Miller J.A., Kalmbach B.E., Radaelli C., Gore B.B., Weed N., Omstead V., Bishaw Y., Shapovalova N.V., Martinez R.A., Fong O., Yao S., Mortrud M., Chong P., Loftus L., Bertagnolli D., Goldy J., Casper T., Dee N., Opitz-Araya X., Cetin A., Smith K.A., Gwinn R.P., Cobbs C., Ko A.L., Ojemann J.G., Keene C.D., Silbergeld D.L., Sunkin S.M., Gradinaru V., Horwitz G.D., Zeng H., Tasic B., Lein E.S., Ting J.T., Levi B.P. Cell Reports, 2021; doi: 10.1016/j.celrep.2021.108754. [ Article ]

  • The SHREAD gene therapy platform for paracrine delivery improves tumor localization and intratumoral effects of a clinical antibody. Smith, S.N., Schubert, R., Simic, B., Brucher, D., Schmid, M., Kirk, N., Freitag, P.C., Gradinaru, V., Pluckthun, A. Proceedings of the National Academy of Sciences, 2021; doi: 10.1073/pnas.2017925118. [ Article ]
  • Multicolor sparse viral labeling and 3D digital tracing of the enteric plexus in mouse proximal colon using a novel adeno-associated virus capsid. Wang, L., Challis, C., Li, S., Fowlkes, C.C., Ravindra Kumar, S., Yuan, P.Q., Tache, Y.F. Neurogastroenterology & Motility, 2020; doi: 10.1111/nmo.14014. [ Article ]

  • Positron emission tomography imaging of novel AAV capsids maps rapid brain accumulation. Seo, J. W., Ingham, E. S., Mahakian, L., Tumbale, S., Wu, B., Aghevlian, S., Shams, S., Baikoghli, M., Jain, P., Ding, X., Goeden, N., Dobreva, T., Flytzanis, N. C., Chavez, M., Singhal, K., Leib, R., James, M. L., Segal, D. J., Cheng, R. H., Silva, E. A., Gradinaru, V., Ferrara, K. W. Nature Communications, 2020; doi: 10.1038/s41467-020-15818-4.  [ Article ]

  • Multiplexed Cre-dependent selection yields systemic AAVs for targeting distinct brain cell types. Ravindra Kumar, S., Miles, T. F., Chen, X., Brown, D., Dobreva, T., Huang, Q., Ding, X., Luo, Y., Einarsson, P. H., Greenbaum, A., Jang, M. J., Deverman, B. E., Gradinaru, V. Nature Methods, 2020; doi: 10.1038/s41592-020-0799-7.  [ Article | Cover | Caltech News Release ]

  • LRP1 is a master regulator of tau uptake and spread. Rauch, J. N., Luna, G., Guzman, E., Audouard, M., Challis, C., Sibih, Y., Leshuk, C., Hernandez, I., Wegmann, S., Hyman, B. T., Gradinaru, V., Kampmann, M., Kosik, K. S. Nature, 2020; doi: 10.1038/s41586-020-2156-5.  [ Article ]

  • RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations. You, S., Yuan, P., Ouellette, B., Zhou, T., Mortrud, M., Balaram, P., Chatterjee, S., Wang, Y., Daigle, T. L., Bosiljka, T., Kuang, X., Gong, H., Luo, Q., Zeng, S., Curtright, A., Dhaka, A., Kahan, A., Gradinaru, V., Chrapkiewicz, R., Schnitzer, M., Zeng, H., Cetin, A. Nature Methods, 2020; doi: 10.1038/s41592-020-0774-3. [ Article | Open access link ]

  • Gut-seeded α-Synuclein fibrils promote gut dysfunction and brain pathology specifically in aged mice. Challis, C., Hori, A., Sampson, T. R., Yoo, B. B., Challis, R. C., Hamilton, A. M., Mazmanian, S. K., Volpicelli-Daley, L. A., Gradinaru, V. Nature Neuroscience, 2020; doi: 10.1038/s41593-020-0589-7.  [ Article | Open access link | Caltech News Release ]

  • A Gut Bacterial Amyloid Promotes α-Synuclein Aggregation and Motor Impairment in Mice. Sampson, T. R., Challis, C., Neha, J., Moiseyenko, A., Ladinsky, M. S., Shastri, G. G., Thron, T., Needham, B. D., Horvath, I., Debelius, J., Janssen, S., Knight, R., Wittung-Stafshede, P., Gradinaru, V., Chapman, M., Mazmanian, S. K. eLife, 2020; doi: 10.7554/eLife.53111.  [ Article ]

  • Tissue Clearing and Its Applications in Neuroscience. Ueda, H. R., Ertürk, A., Chung, K., Gradinaru, V., Chédotal, A., Tomancak, P., Keller, P. J., Nature Reviews Neuroscience, 2020; doi: 10.1038/s41583-019-0250-1.  [ Article ]
  • Imaging neuromodulators with high spatiotemporal resolution using genetically encoded indicators. Patriarchi, T., Cho, J. R., Merten, K., Marley, A., Broussard, G. J., Liang, R., Williams, J., Nimmerjahn, A., von Zastrow, M., Gradinaru, V., Tian, L. Nature Protocols, 2019; doi: 10.1038/s41596-019-0239-2.  [ Article ]

  • Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics. Bedbrook, C. N., Yang, K. K., Robinson, J. E., Mackey, E. D., Gradinaru, V., & Arnold, F. H. Nature Methods, 2019; doi: 10.1038/s41592-019-0583-8.  [ Article | Open access link | Caltech News Release ]

  • Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1. Robinson, J. E., Coughlin, G. M., Hori, A. M., Cho, J. R., Mackey, E. D., Turan, Z., Patriarchi, T., Tian, L., Gradinaru, V. eLife, 2019; doi: 10.7554/eLife.48983.  [ Article ]

  • The Serotonergic Raphe Promote Sleep in Zebrafish and Mice.. Oikonomou, G., Altermatt, M., Zhang, R., Coughlin, G. M., Montz, C., Gradinaru, V., & Prober, D. A. Neuron, 2019; doi: 10.1016/j.neuron.2019.05.038.  [ Article | Video abstract | Caltech News Release ]

  • Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies. Rajendran, P. S., Challis, R. C., Fowlkes, C. C., Hanna, P., Tompkins, J. D., Jordan, M. C., Hiyari, S., Gabris-Weber, B. A., Greenbaum, A., Chan, K. Y., Deverman, B. E., Münzberg, H., Ardell, J. L., Salama, G., Gradinaru, V., Shivkumar, K. Nature Communications, 2019; doi: 10.1038/s41467-019-09770-1.  [ Article ]

  • TRIM9-Mediated Resolution of Neuroinflammation Confers Neuroprotection upon Ischemic Stroke in Mice. Zeng, J., Wang, Y., Luo, Z., Chang, L.-C., Yoo, J. S., Yan, H., Choi, Y., Xie, X., Deverman, B. E., Gradinaru, V., Gupton, S. L., Zlokovic, B. V., Zhao, Z., Jung, J. U. Cell Reports, 2019; doi: 10.1016/j.celrep.2018.12.055.  [ Article ]

  • Systemic AAV vectors for widespread and targeted gene delivery in rodents. Challis, R. C., Kumar, S. R., Chan, K. Y., Challis, C., Beadle, K., Jang, M. J., Kim, H. M., Rajendran, P. S., Tompkins, J. D., Shivkumar, K., Deverman, B. E., Gradinaru, V. Nature Protocols, 2019; doi: 10.1038/s41596-018-0097-3.  [ Article | Cover | Open access link | High resolution fluorescent images ]
  • Whole-Brain Analysis of Cells and Circuits by Tissue Clearing and Light-Sheet Microscopy. Mano, T., Albanese, A., Dodt, H.-U., Erturk, A., Gradinaru, V., Treweek, J. B., Miyawaki, A., Chung, K., Ueda, H. R. The Journal of Neuroscience, 2018, 38(44), 9330–9337; doi: 10.1523/jneurosci.1677-18.2018.  [ Article ]

  • Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors Tommaso Patriarchi, Jounhong Ryan Cho, Katharina Merten, Mark W. Howe, Aaron Marley, Wei-Hong Xiong, Robert W. Folk, Gerard Joey Broussard, Ruqiang Liang, Min Jee Jang, Haining Zhong, Daniel Dombeck, Mark von Zastrow, Axel Nimmerjahn, Viviana Gradinaru, John T. Williams, Lin Tian Science 31 May 2018:eaat4422; doi: 10.1126/science.aat4422.  [ Article | Open access link ]

  • Hydrogel-Tissue Chemistry: Principles and Applications. Gradinaru V, Treweek J, Overton K, Deisseroth K. Annu Rev Biophys. 2018 May 20;47:355-376; doi: 10.1146/annurev-biophys-070317-032905.  [ Article | Open access link ]

  • The Neuropeptide Tac2 Controls a Distributed Brain State Induced by Chronic Social Isolation Stress Moriel Zelikowsky, May Hui, Tomomi Karigo, Andrea Choe, Bin Yang, Mario R. Blanco, Keith Beadle, Viviana Gradinaru, Benjamin E. Deverman and David J. Anderson. Cell 2018 May 17; doi: 10.1016/j.cell.2018.03.037.  [ Article | Open access link | Caltech News Release ]

  • Viral Strategies for Targeting the Central and Peripheral Nervous Systems Bedbrook CN, Deverman BE, Gradinaru V. . Annu Rev Neurosci. 2018 Apr 25. doi: 10.1146/annurev-neuro-080317-062048.  [ Article ]
  • Machine learning to design integral membrane channelrhodopsins for efficient eukaryotic expression and plasma membrane localization. Bedbrook CN, Yang KK, Rice AJ, Gradinaru V, Arnold FH. PLoS Comput Biol. 2017 Oct 23;13(10):e1005786. doi: 10.1371/journal.pcbi.1005786.  [ Article ]

  • Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology. Greenbaum A., Jang M.J., Challis C., Gradinaru V. BMC Biol., 2017; doi: 10.1186/s12915-017-0421-3.  [ Article ] 

  • Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems. Chan, K.Y., Jang, M.J., Yoo, B.B., Greenbaum, A., Ravi, N., Wu., W.L., Sanchez-Guardado, L., Lois, C., Mazmanian, S.K., Deverman, B.E., Gradinaru, V. Nature Neuroscience, 2017; doi:10.1038/nn.4593.  [ Article | Caltech News Release | News and Views | Cover | Open access link ]

  • Global representations of goal-directed behavior in distinct cell types of mouse neocortex. Allen, W.E., Kauvar, I.V., Chen, M.Z., Richman, E.B., Yang, S.J., Chan, K., Gradinaru, V., Deverman, B.E., Luo, L., Deisseroth, K. Neuron, 2017;94, 891-907.  [ Article | DARPA Highlight ]

  • Bone CLARITY: Clearing, imaging, and computational analysis of osteoprogenitors within intact bone marrow. Greenbaum, A.*, Chan, K.Y.*, Dobreva, T., Brown, D., Balani, D.H., Boyce, R., Kronenberg, H.M., McBride, H.J., Gradinaru, V. Sci Transl Med, 2017; 9, eaah6518.  [ Article | Caltech News Release | Nature Research Highlights | Popular Science ]

  • Structure-guided SCHEMA recombination generates diverse chimeric channelrhodopsins. Bedbrook, C.N., Rice, A.J., Yang, K.K., Ding, X., Chen, E., LeProust, E.M., Gradinaru, V., Arnold, F.H. Proc Natl Acad Sci, 2017.  [ Article ]

  • Directed evolution of a bright near-infrared fluorescent rhodopsin using a synthetic chromophore. Herwig, L., Rice, A.J., Bedbrook, C.N., Zhang, R.K., Lignell, A., Cahn, J.K.B., Renata, H., Dodani, S.C., Cho, I., Cai, L., Gradinaru, V., Arnold, F.H. Cell Chem Biol, 2017; doi:10.1016/j.chembiol.2017.02.008.  [ Article | Nature Methods Research Highlights ]
  • Exposing the three-dimensional biogeography and metabolic states of pathogens in cystic fibrosis sputum via hydrogel embedding, clearing, and rRNA labeling. DePas, W.H., Starwart-Lee, R., Van Sambeek, L., Kumar, S.R., Gradinaru, V.*, Newman, D.K.* mBio, 2016; doi:10.1128/mBio.00796-16. [ Article | Caltech News Release | MiPACT Video]

  • Regional glutamine deficiency in tumours promotes dedifferentiation through inhibition of histone demethylation. Pan, M., Reid, M.A., Lowman, X.H., Kulkarni, R.P., Tran, T.Q., Liu, X., Yang, Y., Hernandez-Davies, J.E., Rosales, K.K., Li, H., Hugo, W., Song, C., Xu, X., Schones, D.E., Ann, D.K., Gradinaru, V., Lo, R.S., Locasale, J.W., Kong, M. Nat Cell Biol, 2016; doi:10.1038/ncb3410.  [ Article ]

  • Extracting structural and functional features of widely distributed biological circuits with single cell resolution via tissue clearing and delivery vectors. Treweek, J.B., Gradinaru, V.  Curr Opin Biotechnol, 2016; 40, 193-207.  [ Article ]

  • Single-molecule RNA detection at depth via hybridization chain reaction and tissue hydrogel embedding and clearing. Shah, S.*, Lubeck, E.*, Schwarzkopf, M.*, He, T.*, Greenbaum, A.*, Sohn, C.H., Lignell, A., Choi, H.M.T., Gradinaru, V., Pierce, N.A., Cai, L. Development, 2016; 90.  [ Article ]

  • Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Deverman, B.E.*; Pravdo, P.L.; Simpson, B.P.; Kumar, S.R.; Chan, K.Y.; Banerjee, A.; Wu, W.L.; Yang, B.; Huber, N.; Pasca, S.P.; Gradinaru, V.*, Nat Biotech, 2016; doi:10.1038/nbt.3440.  [ Article | Caltech News Release | Video | CIRM Blog | F1000 Exceptional]

  • Neuroscience: Fluorescent boost for voltage sensors. Gradinaru, V.; Flytzanis, N.C. Nature, 2016; 529, 469-470.  [ Article ]
  • Genomic reconstruction of an uncultured hydrothermal vent gammaproteobacterial methanotroph (family Methylothermaceae) indicates multiple adaptations to oxygen limitation. Skennerton, C.T.; Ward, L.M.; Michel, A.; Metcalfe, K.; Valiente, C.; Mullin, S.; Chan, K.Y.; Gradinaru, V.; Orphan, V.J. Front Microbiol, 2015.  [ Article ] 

  • Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping. Treweek, J.B.; Chan, K.Y.; Flytzanis, N.C.; Yang, B.; Deverman, B.E.; Greenbaum, A.; Lignell, A.; Xiao, C.; Cai, L.; Ladinsky, M.S.; Bjorkman, P.J.; Fowlkes, C.C.; Gradinaru, V. Nat Prot, 2015; doi:10.1038/nprot.2015.122.  [ Article | Troubleshooting ]

  • Genetically encoded spy peptide fusion system to detect plasma membrane-localized proteins in vivo. Bedbrook, C.N.; Kato, M.; Kumar, S.R.; Lakshmanan, A.; Nath, R.D.; Sun, F.; Sternberg, P.W.; Arnold, F.H.; Gradinaru, V. Chem Biol, 2015; doi:10.1016/j.chembiol.2015.06.020.  [ Article | PDF ]

  • Directed evolution of a far-red fluorescent rhodopsin. McIsaac, R.S.; Engqvist, M.K.M.; Wannier, T.; Rosenthal, A.Z.; Herwig, L.; Flytzanis, N.C.; Imasheva, E.S.; Lanyi, J.K.; Balashov, S.P.; Gradinaru, V.; Arnold, F.H. Proc Natl Acad Sci USA 2014; doi: 10.1073/pnas.1413987111.  [ Article ]